Author: Artur Tkachev
Optimizing the Performance of LNP and Liposome Therapeutics with Blood-Free Recombinant Albumin
DMSO-Free Cryopreservation Formulation As An Alternative Cryopreservation Method For Cell-based Therapies
DMSO-Free Cryopreservation of Mesenchymal Stem Cells in Chemically Defined Systems
DMSO-Free Cryopreservation of Human T Lymphocytes in Chemically Defined Systems
Formulation and production of a blood-free and chemically defined virus production media for VERO cells
Evaluation of Transfection Reagents for High Titer Viral Vector Production in the iCELLis Nano Bioreactor
Process Optimization for Lentivirus Production in the iCELLis® Nano Using a Chemically Defined, Animal Component Free HEK293 Media
Abstract Use of FBS in large scale clinical manufacturing applications represents a significant risk due to potential adventitious agent contamination, unreliable supply chain from limited global supply, and considerable variability in vector yield due to lot-to-lot composition variations. To circumvent the many challenges and drawbacks associated with using serum in viral vector production media …
Exbumin – Reconstitution Application Note
OptiVERO – Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media Application Note
Workflow Summary Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify …