OptiPEAK T Lymphocyte XPR Expansion of T Cells without Serum in Chemically Defined Media

Abstract Combine supplements of OptiPEAK T Lymphocyte XPR media. Isolate human T lymphocytes from whole blood. Wash to remove traces of donor serum. Activate and expand T lymphocytes (7-10 days) in OptiPEAK T Lymphocyte XPR serum free media. Harvest T lymphocytes. Perform post- expansion, flow cytometric analysis of phenotypic surface markers. Introduction Human T Cell …

InVitria Expands European and UK Footprint

  InVitria announces an expansion of its global presence to ensure customer satisfaction while offering a local supply for European and UK customers. “We are pleased to announce that InVitria completed qualification of a facility to serve European and UK customers from its operations in Munich, Germany. Our customers in the vaccine, cell and gene …

Chemically Defined iCELLis® Bioreactor-Based Viral Vector Manufacturing – Application of Aber Biomass Probes to Inform Transfection Timing

Abstract Utilization of classical adherent formats in large scale viral vector manufacturing can have significant setbacks due to the lack of scalability of production vessels typically used at small scale. The iCELLis fixed bed bioreactor has emerged as an enabling technology to efficiently scale adherent-based processes in a controlled and highly integrated environment. This technology …

AAV Production – How To Improve Transfection Efficiency and Titer Yield

Production of Adenoviral Associated Viral Vectors   Introduction Adenoviral associated viral (AAV) vectors used in gene therapy have received notable attention considering the recent clinical successes. Traditionally, production of these vectors is achieved by triple transient transfection using the adherent HEK293 cell line in fetal bovine serum (FBS)-supplemented basal media, such as DMEM. The basal …

Blood-Free Fetal Bovine Serum Alternatives in Cell Culture Media

An Interview with Marcus Curl, Director of Product Applications Can you start by providing a quick introduction to InVitria and how you provide value to biopharmaceutical manufacturing? InVitria provides an alternative to serum and serum-proteins in the production and formulation of therapeutics. We specialize in creating completely blood component-free manufacturing environments for gene therapy, cell …

Improvement of Vaccine Manufacturing Process with Use of Recombinant HSA Excipient

Recombinant Human Serum Albumin Excipient – Regulatory Filing and Approval for Use in Vaccine Manufacturing During the 2020 Excipient World Conference & Expo, supported by IPEC-Americas, Kara Quinn, the Associate Director of Engineering for Merck & Co, presented an online seminar detailing the first-hand account of the complex process of regulatory agency filing that led …

Revolutionizing Cell Immunotherapies with Serum Free T Cell Media

CAR T Cell Therapy Shows Promise for Treating Several Applications Pharma Tech Outlook interviewed our CEO Scott Deeter, who shares how InVitria is revolutionizing T cell immunotherapies. InVitria offers the experience and expertise to help you supplement your media of choice with its full line of animal-free, serum-free, recombinant individual cell-culture components. If you would …

Improving Vaccine Development, Performance, and Stability with Recombinant HSA Excipient

Exbumin rHSA Improved Performance and Stability in Vaccine Development   Abstract A key aspect of vaccine production is maintaining stability and function of the final product, including sustained potency and viral titer.  Indeed, this knowledge has driven various preservation strategies, such as lyophilization, temperature control, and localized constitution for use.  However, these are only partial …

Improvements in Viral Vector Manufacturing Process with Chemical Definition of Cell Culture Media & Excipients

Recent Advances of Chemical Definition of Cell Culture Media and Excipients for Virus and Viral Vector Manufacturing We’re excited about the new article published in the Journal of Bioprocessing & Biotechniques:  Recent Advances of Chemical Definition of Cell Culture Media and Excipients for Virus and Viral Vecor Manufacturing: A Review. Abstract Virus production is a …