Lysobac® – Recombinant human lysozyme
Animal component free lysozyme for bacterial lysis, food preservation, and diagnostic applications
Name | Price | Qty | |
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$299.25
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$2,677.50
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Product Description
Lysobac® is InVitria's high-purity recombinant human lysozyme (rHL) produced using the proprietary ExpressTec™ expression platform. As a chemically defined, animal-origin-free alternative to chicken egg-derived lysozyme, Lysobac delivers four times greater activity per milligram for lysing Micrococcus and E. coli, making it the superior choice for bioprocessing, diagnostic applications, and life science research where consistency and performance matter.
Unlike traditional egg-derived lysozyme that introduces batch variability and allergenicity concerns, Lysobac eliminates these risks while delivering enhanced antimicrobial performance. Recent research validates the advantages of recombinant lysozyme production over extraction-based methods, with studies demonstrating improved production strategies in yeast expression systems and enhanced activity profiles compared to chicken-derived alternatives. The identical amino acid sequence to native human lysozyme ensures proper folding and enhanced stability compared to extracted chicken egg lysozyme.
Lysobac addresses critical challenges in bioprocessing workflows where bacterial cell lysis efficiency directly impacts protein recovery yields and process economics. Research demonstrates 5x higher protein recovery from E. coli compared to chicken lysozyme, making it an essential tool for pharmaceutical and biotechnology manufacturing. The rice-based expression system eliminates adventitious agent risks while ensuring consistent enzymatic activity, supporting applications from diagnostic assays to structural biology research.
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Advantages
Enhanced Performance Over Chicken-Derived Lysozyme
- 4x Higher Antimicrobial Activity: Four times more active per milligram than chicken-derived lysozyme for lysing Micrococcus and E. coli, validated in comparative studies
- 5x Higher Protein Recovery: When using equal amounts of Lysobac and chicken lysozyme, 5 times more protein was recovered from E. coli with Lysobac than with chicken lysozyme
- Optimized Human Sequence: Identical amino acid sequence and physiochemical properties to native human lysozyme, ensuring optimal performance in bioprocessing applications
- Enhanced Structural Integrity: Proper protein folding through controlled recombinant production eliminates the degradation seen with extraction-based methods
strong>Research-Validated Applications
- Peer-Reviewed Performance Data: Validated effectiveness across diverse research applications including infectious disease studies, diagnostic assay development, and protein interaction research
- Structural Biology Compatibility: Proven suitability for crystallographic studies and biophysical characterization, with published crystal structure data supporting research applications
- Diagnostic Assay Integration: Demonstrated compatibility with electrochemical detection systems and analytical workflows for pharmaceutical compound analysis
- Pathogen Research Applications: Validated tool for studying bacterial pathogenesis, host-pathogen interactions, and antimicrobial resistance mechanisms
Animal-Origin-Free Manufacturing Excellence
- 100% Chemically Defined: Animal-origin-free (AOF) production eliminates batch variability and regulatory risks associated with egg-derived lysozyme
- Allergen-Free Formulation: Completely free of egg proteins and allergens that can interfere with sensitive assays and downstream applications
- ExpressTec Platform: Rice-based expression system ensures consistent, high-quality protein production without animal-derived material risk
- Controlled Production Process: Eliminates the lot-to-lot variability inherent in extraction-based methods from biological sources
cGMP Manufacturing and Supply Chain Control
- ISO 9001:2015-Certified Facility: Manufactured in InVitria's cGMP-compliant facility in Junction City, Kansas
- Complete Supply Chain Ownership: Vertically integrated manufacturing ensures traceability and quality control from gene to final product
- Scalable Production: Independent of biological donor sources with metric-ton capacity for commercial applications
- Comprehensive Documentation: Full regulatory documentation package supports cGMP workflows and regulatory compliance
Applications
Bioprocessing & Protein Purification
Bacterial Cell Lysis for Recombinant Protein Recovery — Lysobac optimizes the critical bacterial lysis step in recombinant protein purification workflows. The 4x higher activity compared to chicken lysozyme ensures efficient cell wall disruption while the gentle enzymatic action minimizes denaturation of target proteins. Recent advances in recombinant lysozyme production validate the superiority of engineered expression systems over traditional extraction methods for bioprocessing applications. Compatible with defined, animal-origin-free lysis buffers for cGMP manufacturing environments.
Industrial Fermentation Support — Essential tool for recombinant protein expression and bioprocess fermentation, using lysozyme treatment as one of the first steps toward extracting proteins for isolation and purification from bacterial hosts. The enhanced activity profile reduces processing time and buffer volumes while improving overall protein yields. Validated for use in pharmaceutical and biotechnology manufacturing where consistent performance and regulatory compliance are essential.
Diagnostic & Clinical Applications
Advanced Diagnostic Assays — Lysobac enhances microbial lysis efficiency for DNA/RNA extraction and diagnostic workflows. Research demonstrates compatibility with electrochemical detection systems for pharmaceutical compounds including delta-9-tetrahydrocannabinol and methamphetamine analysis. The consistent enzymatic activity ensures reliable performance in clinical diagnostic assays and antimicrobial susceptibility testing. Allergen-free formulation eliminates interference from egg proteins in sensitive analytical methods.
Quality Control Testing — Reliable lysozyme source for pharmaceutical and biotechnology QC applications, supporting microbial testing protocols and assay validation. The controlled recombinant production ensures batch-to-batch consistency critical for regulatory compliance and method validation. Enhanced activity levels reduce testing time while maintaining accuracy and precision in quality control workflows.
Life Science Research
Infectious Disease Research — Validated tool for studying bacterial pathogenesis and host-pathogen interactions. Research applications include Mycobacterium tuberculosis secreted protein screening, Salmonella Typhimurium intestinal engagement studies, and bacterial mechanism investigations. The human lysozyme sequence provides physiological relevance for studying innate immune system function and bacterial resistance mechanisms. Enhanced antimicrobial activity improves experimental sensitivity and reproducibility.
Structural Biology & Protein Science — High purity and consistent structure make Lysobac ideal for crystallographic studies and biophysical characterization. Published crystal structure studies of human lysozyme complexes demonstrate the protein's stability and suitability for detailed structural analysis. Applications include protein interaction studies, enzyme binding research, and biophysical characterization of protein fibrillation and aggregation processes. The controlled production ensures reproducible results in demanding research applications.
Cell Biology & Microbiology Research — Consistent performance for bacterial lysis protocols in academic and industrial research settings. The enhanced activity against gram-positive bacteria supports diverse research applications from basic microbiology to advanced cell biology studies. Compatible with various experimental protocols while maintaining the reproducibility required for research publication and peer review.
Lysobac Technical Specifications
Key specifications for Lysobac as supplied — including purity, endotoxin, formulation, storage, and manufacturing standards. Full Certificate of Analysis available upon request.| Specification | Details |
|---|---|
| Product Name | Lysobac® |
| Brand | InVitria® |
| Catalog Number | 777LYS016 |
| Protein | Recombinant Human Lysozyme |
| Class | Enzyme |
| Synonyms | rhLysozyme, LYZ |
| Expression System | Recombinant, rice-based expression system |
| Molecular Weight | ~16.5 kDa |
| Purity | High-purity recombinant enzyme (see Certificate of Analysis) |
| Biological Activity | Specific Activity ≥ 125,000 units/mg — >4× higher than chicken egg lysozyme |
| Endotoxin Level | See lot-specific Certificate of Analysis |
| Formulation | Lyophilized powder; chemically defined |
| Concentration | Typical working range up to 70,000 U/mL (application-dependent) |
| Appearance | White to off-white lyophilized powder |
| Carrier Protein / Stabilizer | None |
| Sterility | Non-sterile powder; sterile filter after reconstitution if required |
| Packaging | 10 g, 100 g, + scale packaging |
| Storage Conditions | 2–8 °C |
| Shelf Life | 6 years |
| Animal-Origin-Free | Yes (egg- and bovine-free) |
| Production Standard | ISO 9001:2015, cGMP-compliant facility |
| Country of Origin | USA (Junction City, KS) |
Why Select Lysobac Over Egg-Derived Lysozyme or Human-Derived Lysozyme?
| Key Attribute | Lysobac (rHL) | Chicken Egg Lysozyme | Human-Derived Lysozyme |
|---|---|---|---|
| ENHANCED PERFORMANCE | |||
| 4x higher antimicrobial activity per mg | ✅ Yes (Micrococcus & E. coli) | ❌ Baseline activity | ⚠️ Variable by source |
| 5x higher protein recovery from E. coli | ✅ Yes — documented performance | ❌ Standard recovery | ⚠️ Variable performance |
| Identical human lysozyme sequence | ✅ Yes | ❌ Chicken sequence | ✅ Yes |
| Enhanced structural integrity | ✅ Yes — controlled folding | ❌ Extraction-dependent | ⚠️ Source-dependent |
| Research-validated performance | ✅ Multiple peer-reviewed studies | ❌ Limited research validation | ⚠️ Variable documentation |
| SAFETY PROFILE | |||
| Recombinant production | ✅ Yes | ❌ No (egg-derived) | ❌ No (human-derived) |
| Animal-origin-free | ✅ Yes | ❌ Egg-derived | ❌ Human-derived |
| Allergen-free | ✅ Yes (no egg proteins) | ❌ High allergenicity (egg proteins) | ✅ Yes |
| Free of adventitious agent risk | ✅ Yes | ❌ Present (egg source) | ⚠️ Depends on donor screening |
| No donor variability | ✅ Yes | ✅ N/A (non-donor) | ❌ Donor-pool variability |
| TSE/BSE risk | ✅ None (rice-based) | ✅ None | ⚠️ Depends on donor screening |
| FORMULATION ADDITIVES | |||
| Added carrier proteins | ✅ No | ❌ Not typical for lysozyme | ⚠️ Variable by supplier |
| Added preservatives | ✅ No | ⚠️ May contain preservatives | ⚠️ Preservatives required |
| LOT-TO-LOT CONSISTENCY | |||
| Consistent lot-to-lot performance | ✅ Yes — controlled recombinant production | ❌ Variable — extraction yields | ❌ Variable — donor-dependent |
| Performance traceable to a single manufacturing process | ✅ Yes — ExpressTec platform | ❌ Multiple extraction sources | ❌ Multi-donor sources |
| SUPPLY CHAIN AND SCALABILITY | |||
| Scalable production (metric-ton capacity) | ✅ Yes | ⚠️ Limited by egg supply | ❌ Limited by donor availability |
| Supply independent of biological sources | ✅ Yes | ❌ Dependent on egg supply | ❌ Dependent on human donors |
| Scale-up partnership with customer programs | ✅ Yes — InVitria scales with customer programs from research through commercial | ❌ Typically not provided | ❌ Typically not provided |
| Vertically integrated supply chain | ✅ Yes — InVitria owns the complete supply chain | ❌ Multiple suppliers | ❌ Multiple intermediaries |
| Manufactured in United States | ✅ Yes — Junction City, Kansas | Varies by supplier | Varies by supplier |
| Supports animal-free sourcing mandates | ✅ Yes | ❌ No (egg-derived) | ❌ No (human-derived) |
| REGULATORY AND DOCUMENTATION SUPPORT | |||
| ISO 9001:2015 certified manufacturing | ✅ Yes | Varies by supplier | Varies by supplier |
| cGMP-compliant QMS (21 CFR 210/211) | ✅ Yes | Varies by supplier | Varies by supplier |
| Certificate of Origin | ✅ Available | Varies by supplier | Varies by supplier |
| Animal-Free Statement | ✅ Available | ❌ Not applicable (egg-derived) | ❌ Not applicable (human-derived) |
| Comprehensive regulatory support package | ✅ Yes — full documentation for regulatory submissions | ❌ Limited or supplier-dependent | ❌ Complex regulatory pathway |
| Technical support for implementation | ✅ Yes — InVitria applications team supports optimization | ❌ Not typically provided | ❌ Limited |
Lysobac Frequently Asked Questions (FAQs)
Product Overview
What Is Lysobac?
Lysobac® is InVitria's high-purity recombinant human lysozyme (rHL) produced using the proprietary ExpressTec™ rice-based expression platform. As a chemically defined, animal-origin-free alternative to chicken egg-derived lysozyme, Lysobac delivers four times greater activity per milligram for lysing Micrococcus and E. coli, making it the superior choice for bioprocessing, diagnostic applications, and life science research where consistency and performance matter.
What Is Lysobac Made From?
Lysobac is produced recombinantly using InVitria's ExpressTec expression platform, a rice-based, non-mammalian, non-human expression host with no animal-derived, human-derived, or blood-derived materials. Manufacturing is fully animal-origin-free, from raw materials through production equipment to product-contact packaging.
Performance & Activity
Why Is Lysobac More Effective Than Chicken Egg Lysozyme?
Lysobac demonstrates 4x higher antimicrobial activity per milligram compared to chicken lysozyme for lysing Micrococcus and E. coli. When using equal amounts of Lysobac and chicken lysozyme, 5 times more protein was recovered from E. coli with Lysobac than with chicken lysozyme. This enhanced performance is due to its identical amino acid sequence to native human lysozyme and proper protein folding through controlled recombinant production.
How Does Lysobac's Activity Compare to Human-Derived Lysozyme?
Lysobac provides the same human lysozyme sequence and activity as human-derived lysozyme but with consistent lot-to-lot performance and scalable production independent of human donors. The rice-based expression system produces properly folded protein with enhanced stability compared to donor-derived material, while eliminating supply chain limitations and adventitious agent risks.
Regulatory and Quality
Is Lysobac cGMP-Manufactured?
Yes. InVitria manufactures Lysobac under a cGMP-compliant Quality Management System (QMS) with respect to 21 CFR Part 210/211 at its ISO 9001:2015-certified facility in Junction City, Kansas, USA.
What Quality Documentation Is Available for Lysobac?
Lysobac is supported by a Certificate of Analysis, Safety Data Sheet, Guidelines for Use, Certificate of Origin and Animal-Free / TSE-BSE Statement, ISO 9001 certificate, and cGMP Statement. Additional Quality and Regulatory documentation is available on request — including materials for supplier qualification and regulatory filing support.
Does InVitria Provide a Drug Master File (DMF) or Equivalent Regulatory Documentation for Lysobac?
InVitria provides a product-specific Technical Summary for Lysobac containing CMC-style documentation — including manufacturing process, raw material controls, characterization data, release specifications, and lot analysis — designed to support customer regulatory submissions globally, including IND, BLA, and comparable filings under FDA, EMA, and other regulatory authorities. Unlike a Drug Master File, the Technical Summary is provided directly to the customer for incorporation into their own regulatory documentation, giving customers full visibility into the CMC information and supporting global filings without being tied to a single regulatory pathway. The Lysobac Technical Summary is supported by per-lot Certificates of Analysis, Animal-Origin-Free / TSE-BSE statements, ISO 9001 certification, cGMP statements, and change notification under defined policies. Contact us to request the Technical Summary.
Is Lysobac Suitable for Sterile Applications?
Lysobac is supplied as a lyophilized powder and is not classified as sterile. If used in sterile workflows, reconstitute under aseptic conditions and sterile filter before use. This allows integration into sterile bioprocessing and diagnostic applications while maintaining the product's enhanced activity and consistency.
Research Validation
Is Lysobac Validated for Infectious Disease Research?
Yes, Lysobac is proven effective for infectious disease research applications, including studies of bacterial pathogenesis, host-pathogen interactions, and antimicrobial resistance. Research validates its use in studying clinically relevant pathogens including Mycobacterium tuberculosis, Salmonella typhimurium, and other gram-positive bacteria important in human health research.
Can Lysobac Be Used in Diagnostic Applications?
Yes, Lysobac is validated for use in various diagnostic applications including electrochemical detection systems, clinical assays, and analytical workflows. Research demonstrates its compatibility with advanced detection methods for pharmaceutical compounds and its effectiveness in antimicrobial susceptibility testing. The allergen-free formulation eliminates interference from egg proteins in sensitive analytical methods.
What Makes Lysobac Suitable for Structural Biology Research?
Lysobac's high purity and consistent structure make it ideal for crystallographic studies and biophysical characterization. Published crystal structure studies of human lysozyme complexes demonstrate the protein's stability and suitability for detailed structural analysis, protein interaction studies, and biophysical research applications including protein fibrillation and aggregation studies.
Applications and Use
Can Lysobac Be Used in Bioprocessing Applications?
Yes. Lysobac optimizes bacterial cell lysis for recombinant protein recovery from E. coli and other gram-positive bacteria. The 4x higher activity ensures efficient cell wall disruption while the gentle enzymatic action minimizes denaturation of target proteins. Compatible with defined, animal-origin-free lysis buffers for cGMP manufacturing environments.
How Should Lysobac Be Used in Protein Purification?
Use Lysobac at 0.05–0.5 mg/mL (equivalent to ~7,000–70,000 U/mL). Final concentration should be empirically optimized for the target organism and workflow. Typical protocol: resuspend cell pellet in extraction buffer, add Lysobac to desired concentration, incubate according to application requirements, then proceed with downstream processing.
Can Lysobac Replace Chicken Lysozyme in Existing Protocols?
Yes, Lysobac can directly replace chicken lysozyme in most existing protocols. Due to its 4x higher activity, you may need to reduce the concentration accordingly to achieve equivalent lysis. The enhanced performance often allows for reduced incubation times and improved protein recovery yields.
Selection and Comparison
When Should I Choose Lysobac Over Chicken Egg Lysozyme?
Choose Lysobac over chicken lysozyme when your application requires enhanced antimicrobial activity, allergen-free formulation, consistent batch-to-batch performance, or animal-origin-free qualification. Lysobac is particularly advantageous for cGMP manufacturing, sensitive diagnostic assays, and research applications where reproducibility is critical.
When Should I Choose Lysobac Over Human-Derived Lysozyme?
Choose Lysobac when you need scalable production, consistent supply, cost-effective sourcing, or want to eliminate adventitious agent risks associated with human-derived materials. Lysobac provides the same human sequence and activity but with the reliability and documentation of a controlled recombinant manufacturing process.
Technical Support
Is Technical Support Available for Lysobac Implementation?
Yes, InVitria's applications team provides comprehensive technical support for Lysobac implementation, including protocol optimization, troubleshooting, and regulatory guidance for bioprocessing and diagnostic applications. Contact our team for application-specific recommendations and best practices.
Packaging and Ordering
What Packaging Options Are Available for Lysobac?
Lysobac is available in 10 g, 100 g, + scale packaging. Contact InVitria for bulk and custom packaging options.
How Can I Order Lysobac?
Lysobac is available worldwide through multiple purchasing pathways. Most customers work directly with InVitria for full access to the Lysobac product portfolio, bulk and commercial-scale supply, GMP supply commitments, and regulatory documentation support. Lysobac is also available through Fisher Scientific, VWR, and the Labviva procurement platform (which integrates with Ariba, Coupa, Oracle, and Jaggaer) for customers whose institutional procurement systems require integrated supplier ordering. European customers can order with direct fulfillment from InVitria's Rotterdam, Netherlands facility. All customers receive the same product, Certificate of Analysis, and technical support regardless of channel. Contact InVitria or request bulk pricing to place an order.
Storage and Handling
How Should Lysobac Be Stored?
Store Lysobac at -20°C per the Guidelines for Use and product Certificate of Analysis. The lyophilized format provides long-term stability and eliminates cold chain requirements for shipping and short-term storage. Reconstituted solutions should be used promptly or stored according to your specific application requirements.
What Is the Shelf Life of Lysobac?
Lysobac has a 3-year shelf life from date of manufacture when stored properly at -20°C. The lyophilized format ensures stability during shipping and storage, maintaining consistent enzymatic activity throughout the shelf life period.