Leukemia Inhibitory Factor

Product Description

OptiKine™ LIF is InVitria’s recombinant human leukemia inhibitory factor (rhLIF), an animal-origin-free cytokine used in stem cell culture to support self-renewal and help maintain pluripotency. It is designed for researchers working in mouse embryonic stem cell (mESC) culture, feeder-free stem cell systems, and other defined culture workflows where consistent LIF signaling is important.

Unlike conventional rhLIF products produced in bacterial systems, InVitria’s rhLIF is manufactured in a non-animal, non-human expression system and supplied as a sterile liquid for convenient use in the lab. InVitria application data has shown comparable neural stem cell expansion performance to E. coli-derived rhLIF with 500-fold lower bacterial endotoxin, supporting its use in workflows where cleaner culture conditions and reduced contamination risk matter.

Advantages

• Animal-origin-free production
  Manufactured without animal-derived components in a non-animal, non-human expression system, supporting defined stem cell culture workflows.
• Lower bacterial endotoxin
  InVitria application data showed 500-fold lower bacterial endotoxin compared to E. coli-derived rhLIF, with comparable neural stem cell expansion performance.
• Ready-to-use liquid format
  Supplied as a sterile liquid to simplify handling, reduce preparation steps, and avoid reconstitution.
• Supports defined and feeder-free culture strategies
  Useful in workflows where researchers want a cleaner and more reproducible alternative to feeder-dependent culture conditions.
• Manufactured in the United States
  Produced in an ISO 9001:2015-certified facility with direct manufacturer support, helping support consistency, traceability, and supply reliability.

Applications

• Mouse embryonic stem cell (mESC) culture
  Supports pluripotency maintenance and self-renewal in mESC workflows.
• Feeder-free stem cell culture
  Helps support defined culture conditions for researchers looking to reduce reliance on feeder cells.
• Neural stem cell expansion
  Supported by InVitria application data comparing performance against E. coli-derived rhLIF.
• Pluripotent stem cell research
  Useful in workflows where consistent cytokine performance is important for cell behavior and maintenance.
• Developmental biology and regenerative medicine research
  Appropriate for research settings that require animal-origin-free reagents and reproducible culture conditions.

Why Choose OptoKine LIF?

Choose OptiKine rhLIF when your workflow requires more than a generic recombinant cytokine. Key differentiators include animal-origin-free manufacture, production in a non-animal, non-human expression system, a ready-to-use sterile liquid format, and U.S.-based manufacturing. InVitria also provides application data showing comparable neural stem cell expansion performance to E. coli-derived rhLIF with 500-fold lower bacterial endotoxin.

For researchers working in stem cell culture, feeder-free systems, and defined media strategies, these features can help support cleaner culture conditions, simpler handling, and more consistent performance.

Technical Specifications

OptiKine LIF Frequently Asked Questions (FAQs)

What is recombinant human LIF (rhLIF)?
Recombinant human leukemia inhibitory factor is a cytokine used in stem cell culture to support self-renewal and help maintain pluripotency. InVitria’s rhLIF is animal-origin-free and supplied as a sterile liquid.

What does LIF do in stem cell culture?
LIF helps support signaling involved in stem cell maintenance and is commonly used in mouse embryonic stem cell culture and other pluripotent stem cell workflows.

How does InVitria LIF differ from E. coli-derived rhLIF?
InVitria application data showed comparable neural stem cell expansion performance with 500-fold lower bacterial endotoxin than E. coli-derived rhLIF.

Is InVitria LIF animal-origin-free?
Yes. The product page and related use-guideline content describe it as animal-origin-free and suited for defined stem cell workflows.

Can this product be used in feeder-free culture systems?
Yes. The current product page positions it for feeder-free systems, especially in stem cell applications where researchers want cleaner and more reproducible conditions.

What applications is InVitria LIF used for?
Common applications include mESC culture, feeder-free stem cell culture, neural stem cell expansion, pluripotent stem cell research, and developmental biology research.

Is the product supplied as a liquid or a powder?
The live page lists the product form as liquid and describes it as a ready-to-use sterile liquid formulation.

What technical documents are available?
The page currently offers bulk quote requests, CoA requests, a data sheet, and LIF guidelines for use.

Published Research Citing OptiKine LIF

The publications below report use of OptiKine LIF across a range of applications. In some cases, the material is cited under distributor or private-label catalog numbers, or as a component of formulated products.

Full bibliography available upon request. Contact us for the complete publication list.