Blood-Free Cell Culture Media

InVitria Complete Media: optimized, reliable, scalable performance for therapeutic applications

Cultured cells and cell products destined for cell and gene therapy or other clinical cGMP applications must meet the highest standards for manufacturing quality, safety, performance, consistency, and efficiency. InVitria has carefully optimized its animal-free, serum-free, chemically defined media formulations for clinically and industrially significant cultured cell lines. InVitria’s product line eliminates the guesswork, making the transition to serum-free culture quick and easy.

Performance. Defined.

Primary cells and immortalized cell lines need more than just nutrients and salts to thrive ex vivo; they also require the presence of key extracellular proteins like transferrin for iron management, insulin for nutrient uptake, and albumin to support optimal cellular health and growth.

InVitria Media satisfies these cellular requirements with fully recombinant proteins that are human in sequence, fully free of animal components and produced in the US, using cGMP standards in an ISO 9001:2015 certified facility. Reducing or eliminating fetal bovine serum (FBS) from cell culture:

• Reduces or eliminates the introduction of undesirable viral RNA and DNA, prion proteins, undefined antigenic molecules, and human or bovine cytokines and hormones.
• Provides better, more consistent, more scalable cell growth and performance in culture, and more dependable manufacturing yield, product validation, and clinical safety of downstream clinical products.
• Reduces foaming and other technical challenges in bioreactors.
• Eliminates concerns about the fluctuating global agricultural supply chain and limitations on availability of bovine serum.

GMP Complete Media:

OptiVERO Complete Media: optimized for expansion and maintenance of VERO cells in tissue culture flask and 3D plastic microcarriers. A regulatory-friendly solution for producing clinically relevant viral particles during vaccine manufacturing (e.g., attenuated Dengue, Influenza and Zika viruses).

At a Glance: InVitria chemically defined cell culture media

*All protein components included in InVitria’s media products are fully human in sequence, fully recombinant, and expressed in a non-animal host. All processing and packaging steps are fully free of animal components and are produced in the US, using cGMP standards in an ISO 9001:2015 certified facility.

Related Resources

Resource Type	Title	Cell Lines	Relevance
Application Note	OptiVERO – Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media Application Note	VERO	VERO virus production in 2D flask culture with chemically defined media.
Application Note	OptiVERO – Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media	VERO	VERO virus production on microcarriers with chemically defined media.
Application Note	OptiVERO – Expansion of VERO Cells Without Serum in Chemically Defined Cell Culture Media	VERO	VERO cell expansion in chemically defined media.
White Paper	VERO Expansion in the iCellis® Bioreactor with Chemically Defined and Blood Free Virus Production Media	VERO	VERO expansion in iCELLis bioreactor with chemically defined media.
Poster	Formulation of a Blood-Free and Chemically Defined Virus Production Media for VERO Cells Poster	VERO	Chemically defined VERO virus production media compared to FBS and VP-SFM.
Poster	Advancing Vaccine Excellence	VERO	How blood-free media supports vaccine manufacturing.
Journal Articles (DOI Links)	Formulation and Production of a Blood-free and Chemically Defined Virus Production Media for VERO Cells	VERO	Peer-reviewed publication on chemically defined VERO virus production media.
Webinar	Accelerating Vaccine Development Through Use of Blood-Free Components	—	Webinar on blood-free media in vaccine development.