Blood-Free Cell Culture Media

InVitria Complete Media: optimized, reliable, scalable performance for therapeutic applications

Cultured cells and cell products destined for cell and gene therapy or other clinical cGMP applications must meet the highest standards for manufacturing quality, safety, performance, consistency, and efficiency. InVitria has carefully optimized its animal-free, serum-free, chemically defined media formulations for clinically and industrially significant cultured cell lines. The InVitria product line takes the guesswork out of transitioning quickly and painlessly to serum-reduced or serum-free cell culture for your RUO or cGMP needs.

Performance. Defined.

Primary cells and immortalized cell lines need more than just nutrients and salts to thrive ex vivo; they also require the presence of key extracellular proteins like transferrin for iron management, insulin for nutrient uptake, and albumin to support optimal cellular health and growth.

InVitria Media satisfies these cellular requirements with fully recombinant proteins that are human in sequence, fully free of animal components, and are produced in the US, using cGMP standards in an ISO 9001:2015 certified facility. Reducing or eliminating fetal bovine serum (FBS) from cell culture:

• Reduces or eliminates the introduction of undesirable viral RNA and DNA, prion proteins, undefined antigenic molecules, as well as human or bovine cytokines and hormones.
• Provides better, more consistent, more scalable cell growth and performance in culture, and more dependable manufacturing yield, product validation, and clinical safety of downstream clinical products.
• Reduces foaming and other technical challenges in bioreactors.
• Eliminates concerns about the fluctuating global agricultural supply chain and limitations on availability of bovine serum.

GMP Complete Media:

OptiVERO Complete Media: optimized for expansion and maintenance of VERO cells in tissue culture flask and 3D plastic microcarriers. Regulatory-friendly option for production of clinically relevant viral particles during vaccine manufacturing (e.g., attenuated Dengue, Influenza and Zika viruses).

The OptiPEAK Platform Media line:

OptiPEAK HEK293 Platform Media: optimized to promote superior expansion kinetics of HEK293, both in tissue culture and in scalable bioreactor format for clinical and commercial manufacturing. This media is ideal for cell growth as well as for regulatory-friendly production of adeno-associated viral vectors (AAV) and lentiviral vectors for gene therapy applications. In development: optimization for HEK293 suspension culture.

OptiPEAK T-lymphocyte Platform Media: optimized for expansion of T cell subsets after isolation from whole blood; regulatory-friendly option for clinical manufacturing of cells for CAR-T or other T-cell-based adoptive cell transfer applications. Yields consistent expansion of T cells comparable to cells in 5% human serum and greater than cells in 10% fetal bovine serum, with CD4+ and CD8+ ratios comparable to cells grown in human and bovine serum.

OptiPEAK Media Optimization Services

Let InVitria help you discover how much farther you can push cell growth, cell performance, expansion of primary-cell subsets and phenotypes, or viral titer in serum-reduced or serum-free culture. Starting with our pre-formulated platform media, InVitria offers the experience and know-how to help you take advantage of its full line of animal-free, serum-free, recombinant individual cell-culture components. Our Product Applications team is ready to help you build a serum-reduced or chemically defined medium optimized to your desired cell type and application.

InVitria Custom Media Formulation Services

Have specific needs? To learn more about transitioning your cell culture system to complete chemically defined serum-free media, contact us or follow the link to InVitria’s Custom Media Formulation services page.

At a Glance: InVitria chemically defined cell culture media

*All protein components included in InVitria’s media products are fully human in sequence, fully recombinant, and expressed in a non-animal host. All processing and packaging steps are fully free of animal components and are produced in the US, using cGMP standards in an ISO 9001:2015 certified facility.