ITSE+A™ – Blood-free cell culture media supplement
Blood-free cell culture media supplement and serum reducer
Product Description
ITSE + A™ (Albumin) (ITSE+A) is a 100x liquid recombinant cell culture supplement containing insulin, transferrin, selenium, ethanolamine, and recombinant human serum albumin (rHSA) — the most complete serum-replacement supplement in InVitria's ITS product family. The 5-component formulation delivers 1.00 g/L insulin, 0.55 g/L transferrin, 0.67 mg/L selenium, 0.20 g/L ethanolamine, and 20.00 g/L recombinant human serum albumin in a chemically defined, animal-origin-free profile suitable for serum-free and reduced-serum workflows across CAR-T cell manufacturing, biopharmaceutical production, vaccine manufacturing, and regenerative medicine applications.
ITSE+A extends the 4-component ITSE supplementation profile with the addition of recombinant human serum albumin — a functionally critical protein that stabilizes growth factors, transports lipids and fatty acids, buffers oxidative stress, and binds metal ions across serum-free culture systems. The inclusion of rHSA differentiates ITSE+A from 3-component ITS supplements (insulin/transferrin/selenium) and 4-component ITSE supplements (ITS plus ethanolamine), delivering a more complete serum-replacement profile for demanding cell types and production workflows. Unlike competing 5-component ITS+BSA supplements that rely on bovine serum albumin — introducing animal-derived raw material considerations, pooled plasma variability, and adventitious agent qualification requirements — ITSE+A is formulated with recombinant human serum albumin, eliminating plasma- and bovine-derived raw materials in the albumin component and supporting ICH Q5A-aligned regulatory pathways for cell therapy, gene therapy, and biologics programs.
ITSE+A has been used in peer-reviewed biopharmaceutical research including CAR-T cell manufacturing process development. Lamas et al. (2022) at AstraZeneca/MedImmune used ITSE+A serum replacement supplement to support primary T-cell culture in X-VIVO 15 media during CAR-T manufacturing process development in AMBR 15 bioreactors, published in Biotechnology Progress (DOI: 10.1002/btpr.3275). Lien et al. (2023) incorporated ITSE+A in HEK293A serum-free workflows for coxsackievirus A10 vaccine production, published in Virus Research (DOI: 10.1016/j.virusres.2023.199101). ITSE+A cross-references with ITS Animal-Free™ (3-component) and ITSE Animal-Free™ (4-component) within InVitria's ITS product family, providing a range of defined supplementation options for serum-free and chemically defined media development. ITSE+A is manufactured under an ISO 9001:2015-certified, cGMP-compliant quality management system and is available in 10 mL, 100 mL, and 10 mL × 10 case formats. Full regulatory documentation — including Certificate of Analysis, Safety Data Sheet, Animal-Free Statement, and cGMP documentation — is available on request.
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Advantages
Validated in CAR-T Cell Manufacturing Process Development
- ITSE+A™ serum replacement supplement was used by Lamas et al. (2022) at AstraZeneca/MedImmune to support primary T-cell culture in X-VIVO 15 media during CAR-T manufacturing process development in AMBR 15 bioreactors, published in Biotechnology Progress (DOI: 10.1002/btpr.3275).
- This establishes ITSE+A as a cell culture supplement with demonstrated use in industrial CAR-T manufacturing workflows — supporting the T-cell expansion requirements specific to autologous and allogeneic cell therapy programs.
- The 5-component defined composition (insulin, transferrin, selenium, ethanolamine, and recombinant human serum albumin) delivers the protein stabilization, iron delivery, and membrane biosynthesis support required for T-cell expansion in serum-free and reduced-serum media systems.
The Most Complete Supplement in the ITS Family
- ITSE+A extends the 4-component ITSE profile with the addition of recombinant human serum albumin at 20.00 g/L, delivering the most comprehensive serum-replacement supplement in InVitria's ITS product family — providing insulin, transferrin, selenium, ethanolamine, and rHSA in a single 100x liquid concentrate.
- The recombinant human serum albumin component stabilizes growth factors, transports lipids and fatty acids, buffers oxidative stress, and binds metal ions — addressing the protein stabilization and carrier functions that ITS Animal-Free™ (3-component) and ITSE Animal-Free™ (4-component) do not cover.
- The 5-component format is particularly well-suited for CAR-T manufacturing, stem cell culture, vaccine production, and biopharmaceutical workflows where comprehensive serum-replacement is the process goal.
Fully Recombinant Human Albumin — Not Bovine BSA
- Unlike competing 5-component ITS+BSA supplements that rely on bovine serum albumin, ITSE+A is formulated with recombinant human serum albumin — eliminating the animal-derived raw material considerations, pooled bovine plasma variability, and adventitious agent qualification requirements associated with BSA-containing supplements.
- The recombinant human albumin component aligns ITSE+A with the ICH Q5A-aligned regulatory pathways required for cell therapy, gene therapy, and biologics programs where elimination of animal-derived raw materials is a CMC and regulatory priority.
- Human-sequence albumin avoids the cross-species protein interactions and immunogenicity considerations that can accompany bovine serum albumin use in human therapeutic cell culture workflows.
Regulatory-Ready Documentation and Manufacturing
- ITSE+A is manufactured under an ISO 9001:2015-certified, cGMP-compliant quality management system.
- Complete regulatory package available: Certificate of Analysis, Safety Data Sheet, Guidelines for Use, Certificate of Origin and Animal-Free / TSE-BSE Statement, ISO 9001 certificate, and cGMP Statement.
- Endotoxin controlled to ≤ 3.00 EU/mL, tested and released on every lot, with full traceability — supporting ICH Q5A-aligned regulatory pathways for biopharmaceutical, cell therapy, and gene therapy submissions.
Applications
Stem Cell Expansion and Pluripotent Stem Cell Culture. ITSE+A™ supports serum-free and reduced-serum culture of pluripotent stem cells (hESC, iPSC), mesenchymal stem cells (MSCs), and adult stem/progenitor cell populations where comprehensive defined supplementation — insulin, transferrin, selenium, ethanolamine, and recombinant human serum albumin — is required for self-renewal, maintenance, and directed differentiation. The inclusion of rHSA provides the protein stabilization and fatty acid transport functions critical for stem cell expansion in chemically defined media systems, while the ethanolamine component supports membrane biosynthesis in the actively proliferating populations characteristic of stem cell culture. The animal-origin-free composition aligns with the translational requirements of regenerative medicine and cell therapy programs where downstream clinical application demands elimination of bovine- and human-derived raw materials.
CAR-T and T-Cell Manufacturing. ITSE+A is validated in CAR-T cell manufacturing process development workflows. Lamas et al. (2022) at AstraZeneca/MedImmune used ITSE+A serum replacement supplement to support primary T-cell culture in X-VIVO 15 media during CAR-T manufacturing process development in AMBR 15 bioreactors, published in Biotechnology Progress (DOI: 10.1002/btpr.3275). The 5-component composition provides the protein stabilization, iron delivery, membrane biosynthesis support, and albumin-mediated fatty acid transport required for T-cell activation, expansion, and transduction workflows — supporting both autologous and allogeneic cell therapy manufacturing at research, process development, and clinical scales. The defined, animal-origin-free profile simplifies raw material qualification for IND/BLA-stage cell therapy programs where CMC documentation and regulatory pathway considerations drive supplement selection.
Vaccine and Viral Vector Production. ITSE+A supports serum-free viral vector and vaccine production workflows in HEK293, VERO, and related manufacturing cell lines. Lien et al. (2023) incorporated ITSE+A in HEK293A serum-free culture for coxsackievirus A10 vaccine production, published in Virus Research (DOI: 10.1016/j.virusres.2023.199101). The rHSA component stabilizes viral vectors and vaccine antigens during production and downstream processing, while the defined insulin-transferrin-selenium-ethanolamine profile supports the high-density cell culture conditions required for commercial-scale viral vector and vaccine manufacturing. The animal-origin-free composition aligns with regulatory expectations for elimination of adventitious agent risk in vaccine and gene therapy manufacturing.
Biopharmaceutical Cell Line Development and Recombinant Protein Production. ITSE+A is compatible with serum-free suspension culture of industrially relevant biopharmaceutical production cell lines including CHO, NS0, HEK293, and hybridoma systems used in monoclonal antibody, recombinant protein, and biosimilar manufacturing. The 5-component supplementation profile provides comprehensive serum replacement for cell line development, process development, and production-scale workflows — supporting high-density culture, productivity, and product quality in chemically defined and serum-free media formulations. The rHSA component stabilizes expressed proteins against shear stress and aggregation during bioreactor culture, while the defined insulin-transferrin-selenium-ethanolamine base supports the metabolic and growth requirements of industrially optimized production cell lines.
Serum-Free and Chemically Defined Media Formulation. Beyond its published applications, ITSE+A serves as a foundational serum-replacement supplement in the formulation of fully chemically defined media systems across mammalian cell culture. At the industry-standard 100x concentration, the product provides a consistent source of insulin (1.00 g/L), transferrin (0.55 g/L), selenium (0.67 mg/L), ethanolamine (0.20 g/L), and recombinant human serum albumin (20.00 g/L) — delivering the most comprehensive single-addition serum replacement in InVitria's ITS product family. Typical use is at 1x working concentration for routine mammalian cell culture, with adjustments available for cell types or production systems requiring different supplementation profiles. ITSE+A is compatible with common basal media including DMEM, DMEM/F-12, RPMI 1640, X-VIVO 15, and proprietary serum-free formulations, and cross-references with ITS Animal-Free™ (3-component) and ITSE Animal-Free™ (4-component) within InVitria's ITS product family to support a full range of defined media development needs.
ITSE+A Animal-Free Technical Specifications
Key specifications for ITSE+A as supplied — including purity, endotoxin, formulation, storage, and manufacturing standards. Full Certificate of Analysis available upon request.| Specification | Details |
|---|---|
| Product Name | ITSE+A™ |
| Brand | InVitria® |
| Catalog Number | 777ITS092 |
| Protein | Recombinant cell culture media supplement with ethanolamine and rHSA |
| Class | Cell Culture Supplement with Albumin |
| Synonyms | ITSE+A AF |
| Expression System | Recombinant, rice-based expression system |
| Endotoxin Level | ≤ 3.0 EU/mL |
| Formulation | rh-Insulin 1.00 g/L; rh-Transferrin 0.55 g/L; Selenium 0.00067 g/L; Ethanolamine 0.20 g/L; rh-Albumin 20.00 g/L |
| Concentration | 100× sterile concentrate (frozen); dilute 10 mL into 1 L basal media for 1× use |
| Appearance | Clear to slightly opalescent, colorless to slightly yellow liquid (frozen) |
| Carrier Protein / Stabilizer | None (no additives or preservatives) |
| Sterility | Sterile |
| Packaging | 10 mL, 100 mL, 10 × 10 mL, + scale packaging |
| Storage Conditions | –20 °C (frozen) |
| Shelf Life | 2 years |
| Animal-Origin-Free | Yes |
| Production Standard | ISO 9001:2015, cGMP-compliant facility |
| Country of Origin | USA (Junction City, KS) |
Why Select ITSE+A AF Over Plasma-Derived ITSE?
| Key Attribute | ITSE+A Animal-Free | Plasma-Derived ITSE |
|---|---|---|
| COMPOSITION AND FORMAT | ||
| 100x sterile liquid concentrate — ready to dilute into basal media | ✅ Yes | ✅ Yes |
| Insulin concentration (at 100x) | ✅ 1.0 g/L recombinant human insulin | ⚠️ 1.0 g/L — industry standard, but source varies (recombinant or bovine) |
| Transferrin concentration (at 100x) | ✅ 0.55 g/L recombinant human transferrin | ⚠️ 0.55 g/L — but isolated from human or bovine blood plasma |
| Selenium concentration (at 100x) | ✅ 0.67 mg/L sodium selenite | ⚠️ 0.5–0.67 mg/L — sodium selenite or selenious acid (varies by supplier) |
| Ethanolamine concentration (at 100x) | ✅ 0.20 g/L | ⚠️ 0.20 g/L — industry standard, but sourcing varies by supplier |
| Recombinant human albumin (rHSA) (at 100x) | ✅ 20.00 g/L | ❌ No — bovine serum albumin (BSA), typically 2.5–5 g/L |
| FORMULATION ADVANTAGE | ||
| Supports CAR-T cell manufacturing workflows | ✅ Yes — used in peer-reviewed CAR-T process development (Lamas et al., 2022) | ⚠️ Variable — bovine-derived content creates IND/BLA qualification burden |
| Supports lipid-dependent cell types (iPSC, CHO stress) | ✅ Yes — ethanolamine + rHSA support membrane biosynthesis and fatty acid transport | ⚠️ Variable |
| SAFETY PROFILE | ||
| Recombinant production | ✅ Yes | ❌ No (plasma- and bovine-derived) |
| Animal-origin-free | ✅ Yes | ❌ Blood-derived |
| Blood-free | ✅ Yes | ❌ Blood-derived |
| Free of adventitious agent risk | ✅ Yes | ❌ Adventitious agent risk present |
| No donor variability | ✅ Yes | ❌ Donor-pool variability |
| TSE/BSE risk | ✅ None (no animal source) | ❌ Elevated — bovine BSA carries direct TSE/BSE risk |
| LOT-TO-LOT CONSISTENCY | ||
| Consistent lot-to-lot performance | ✅ Yes — controlled recombinant production | ❌ Variable — source-dependent |
| Performance traceable to a single manufacturing process | ✅ Yes — ExpressTec platform | ❌ Multi-source, multi-donor, or multi-supplier |
| SUPPLY CHAIN AND SCALABILITY | ||
| Scalable production (metric-ton capacity) | ✅ Yes | ❌ Limited by plasma donor supply |
| Supply independent of blood donation | ✅ Yes | ❌ Dependent on donor supply |
| Scale-up partnership with customer programs | ✅ Yes — InVitria scales with customer programs from clinical through commercial | ❌ Typically not provided |
| Vertically integrated supply chain | ✅ Yes — InVitria owns the complete supply chain | ❌ Multiple intermediaries |
| Manufactured in United States | ✅ Yes — Junction City, Kansas | Varies by supplier |
| Supports animal-free and sustainable sourcing mandates | ✅ Yes | ❌ No |
| REGULATORY AND DOCUMENTATION SUPPORT | ||
| ISO 9001:2015 certified manufacturing | ✅ Yes | Varies by supplier |
| cGMP-compliant QMS (21 CFR 210/211) | ✅ Yes | Varies by supplier |
| Certificate of Origin | ✅ Available | Varies by supplier |
| Animal-Free Statement | ✅ Available | ❌ Not applicable |
| Regulatory support package | ✅ Yes — full dossier-quality documentation for IND and BLA submissions | ❌ Limited or supplier-dependent |
| Technical support for regulatory filings | ✅ Yes — InVitria technical team supports customer regulatory submissions | ❌ Not typically provided |
ITSE+A™ Frequently Asked Questions (FAQs)
Product Overview
What Is ITSE+A?
ITSE+A (catalog 777ITS092) is a chemically defined, animal-origin-free cell culture supplement supplied as a sterile 100x liquid concentrate containing insulin (1.00 g/L), recombinant human transferrin (0.55 g/L), selenium as sodium selenite (0.67 mg/L), ethanolamine (0.20 g/L), and recombinant human serum albumin (20.00 g/L). ITSE+A is the most complete serum-replacement supplement in InVitria's ITS product family — a 5-component defined formulation validated in CAR-T cell manufacturing process development and designed for workflows requiring comprehensive serum replacement in chemically defined and serum-free media systems.
What Is ITSE+A Made From?
ITSE+A is formulated with recombinant proteins and chemically defined components in a fully animal-origin-free profile. The recombinant human transferrin and recombinant human serum albumin components eliminate plasma- and bovine-derived raw materials, and the product is manufactured without any animal-derived components in raw materials, processing aids, or manufacturing equipment — supporting ICH Q5A-aligned compliance and simplifying TSE/BSE qualification for regulatory submissions.
Composition and Specifications
What Is the Composition of ITSE+A?
ITSE+A is supplied as a 100x liquid concentrate with the following composition: insulin at 1.00 g/L, recombinant human transferrin at 0.55 g/L, selenium (as sodium selenite) at 0.67 mg/L, ethanolamine at 0.20 g/L, and recombinant human serum albumin at 20.00 g/L. This 5-component formulation extends the ITSE supplementation profile with the addition of recombinant human serum albumin, delivering the most comprehensive single-addition serum replacement in InVitria's ITS product family.
What Does the "+A" in ITSE+A Stand For?
The "+A" indicates the addition of recombinant human serum albumin (rHSA) to the base ITSE (insulin-transferrin-selenium-ethanolamine) supplementation profile. The rHSA component provides protein stabilization, lipid and fatty acid transport, oxidative stress buffering, and metal ion binding functions that the ITSE formulation alone does not cover — making ITSE+A particularly well-suited for workflows where comprehensive serum replacement is the process goal.
What Is the Endotoxin Specification for ITSE+A?
ITSE+A is released at ≤3.00 EU/mL, tested on every lot. Endotoxin is controlled through raw material sourcing and manufacturing process controls, with full traceability from raw materials through finished product.
Why Does ITSE+A Need to Be Stored Frozen?
ITSE+A is stored at -20°C (frozen) to preserve the stability and activity of the recombinant human serum albumin component over the product's shelf life. The rHSA content is the primary driver of the frozen storage requirement, differentiating ITSE+A's storage conditions from the refrigerated storage specifications (2–8°C) of the 3-component ITS Animal-Free™ and 4-component ITSE Animal-Free™ supplements.
Regulatory and Quality
Is ITSE+A cGMP-Manufactured and Animal-Origin-Free?
ITSE+A is manufactured under an ISO 9001:2015-certified, cGMP-compliant quality management system. The product is 100% animal-origin-free — raw materials, the recombinant protein components, manufacturing equipment, and product-contact packaging are all free of animal-derived components.
What Quality Documentation Is Available for ITSE+A?
ITSE+A is supported by a Certificate of Analysis, Safety Data Sheet, Guidelines for Use, Certificate of Origin and Animal-Free / TSE-BSE Statement, ISO 9001 certificate, and cGMP Statement. Additional Quality and Regulatory documentation is available on request, including materials for IND-supporting packages and supplier qualification.
Does ITSE+A Support ICH Q5A Compliance?
Yes. The recombinant human transferrin and recombinant human serum albumin components are produced without animal-derived materials, aligning with ICH Q5A guidance on viral safety evaluation of biotechnology products derived from cell lines. The absence of plasma-derived transferrin and bovine serum albumin eliminates the compounded adventitious agent qualification burden associated with plasma- and animal-sourced raw materials, simplifying the CMC documentation required for biopharmaceutical, cell therapy, and gene therapy programs.
Research Validation
Has ITSE+A Been Used in CAR-T Cell Manufacturing?
Yes. Lamas et al. (2022) at AstraZeneca/MedImmune used ITSE+A serum replacement supplement to support primary T-cell culture in X-VIVO 15 media during CAR-T manufacturing process development in AMBR 15 bioreactors, published in Biotechnology Progress (DOI: 10.1002/btpr.3275). This establishes ITSE+A as a cell culture supplement with demonstrated use in industrial CAR-T manufacturing process development — supporting the T-cell expansion, activation, and transduction requirements specific to autologous and allogeneic cell therapy programs.
Has ITSE+A Been Used in Vaccine Production?
Yes. Lien et al. (2023) incorporated ITSE+A in HEK293A serum-free culture for coxsackievirus A10 vaccine production, published in Virus Research (DOI: 10.1016/j.virusres.2023.199101). The 5-component defined composition supports serum-free viral vector and vaccine manufacturing workflows in HEK293, VERO, and related production cell lines.
Selection and Comparison
What Is the Difference Between ITSE+A and ITSE AF?
ITSE Animal-Free™ is a 4-component supplement containing insulin, transferrin, selenium, and ethanolamine. ITSE+A™ is a 5-component supplement that adds recombinant human serum albumin (rHSA) at 20.00 g/L as a fifth component. For workflows where albumin supplementation is required — including CAR-T cell manufacturing, stem cell expansion, and comprehensive serum replacement applications — ITSE+A is the appropriate product. For standard chemically defined and serum-free media formulation where albumin is supplied separately or not required, ITSE AF is sufficient. Contact InVitria to discuss the best option for your specific application.
What Is the Difference Between ITSE+A and ITS AF?
ITS Animal-Free™ is a 3-component supplement containing insulin, transferrin, and selenium. ITSE+A™ is a 5-component supplement that adds both ethanolamine (for phospholipid biosynthesis support) and recombinant human serum albumin (for protein stabilization and fatty acid transport). The two products serve different levels of defined supplementation: ITS AF for minimal defined supplementation in workflows where ethanolamine and albumin are supplied separately or not required, and ITSE+A for comprehensive serum replacement in demanding cell culture workflows. Contact InVitria to discuss the best option for your specific application.
How Is ITSE+A Different From ITS+BSA Supplements?
ITSE+A is formulated with recombinant human serum albumin (rHSA), while competing 5-component ITS+BSA supplements rely on bovine serum albumin (BSA). The difference matters for three reasons: rHSA eliminates the bovine-derived raw material considerations, pooled bovine plasma variability, and adventitious agent qualification requirements associated with BSA-containing supplements; human-sequence albumin avoids the cross-species protein interactions that can accompany bovine albumin use in human therapeutic cell culture; and rHSA aligns with ICH Q5A regulatory pathways for cell therapy, gene therapy, and biologics programs where elimination of animal-derived raw materials is a CMC and regulatory priority.
Is ITSE+A Equivalent to Plasma-Derived ITS+BSA Supplements?
ITSE+A provides 5-component defined supplementation functionally equivalent to plasma-derived and bovine-based ITS+BSA supplements at the same 100x concentration. The critical differences are raw material sourcing and regulatory profile: ITSE+A uses recombinant human transferrin and recombinant human serum albumin, while plasma-derived ITS+BSA supplements rely on plasma-sourced transferrin and bovine serum albumin. The recombinant sources eliminate the compounded supply chain, adventitious agent qualification, and regulatory considerations associated with plasma- and bovine-derived raw materials.
Application and Use
At What Concentration Should ITSE+A Be Used?
ITSE+A is typically used at 1x working concentration (1:100 dilution of the 100x concentrate) for routine mammalian cell culture. Working concentration adjustments may be appropriate for specific cell types, culture systems, or production workflows. Actual working concentration should be determined empirically for each cell line and application.
Which Cell Types Is ITSE+A Compatible With?
ITSE+A is compatible with serum-free and reduced-serum culture of a broad range of mammalian cell types including CHO, HEK293, VERO, NS0, hybridoma, primary T cells (including CAR-T manufacturing workflows), human pluripotent stem cells (hESC, iPSC), mesenchymal stem cells (MSCs), and adult stem/progenitor cell populations. The 5-component composition — with the addition of recombinant human serum albumin — is particularly well-suited for cell types and workflows requiring comprehensive protein stabilization, fatty acid transport, and oxidative stress buffering.
Which Basal Media Is ITSE+A Compatible With?
ITSE+A is compatible with common basal media including DMEM, DMEM/F-12, RPMI 1640, X-VIVO 15, and proprietary serum-free formulations. The product is typically added directly to basal medium at 1x working concentration as part of a complete defined or serum-free formulation. Lamas et al. (2022) used ITSE+A with X-VIVO 15 media specifically in CAR-T cell manufacturing process development.
Can ITSE+A Be Used for CAR-T Cell Manufacturing?
Yes. ITSE+A has been used by Lamas et al. (2022) at AstraZeneca/MedImmune to support primary T-cell culture during CAR-T manufacturing process development in AMBR 15 bioreactors. The 5-component composition provides the protein stabilization, iron delivery, membrane biosynthesis support, and albumin-mediated fatty acid transport required for T-cell activation, expansion, and transduction workflows. The defined, animal-origin-free profile simplifies raw material qualification for IND/BLA-stage cell therapy programs.
Packaging and Ordering
What Packaging Options Are Available for ITSE+A?
ITSE+A is available in 10 mL and 100 mL liquid formats, as well as a 10 mL × 10 case configuration for higher-volume workflows. Contact InVitria for bulk and custom packaging options.
How Can I Order ITSE+A?
ITSE+A is available worldwide through multiple purchasing pathways. Most customers work directly with InVitria for full access to the ITSE+A product portfolio, bulk and commercial-scale supply, favorable volume-tier pricing, faster supply chain turnaround, GMP supply commitments, and regulatory documentation support. ITSE+A is also available through Fisher Scientific, VWR, and the Labviva procurement platform (which integrates with Ariba, Coupa, Oracle, and Jaggaer) for customers whose institutional procurement systems require integrated supplier ordering. European customers can order with direct fulfillment from InVitria's Rotterdam, Netherlands facility. All customers receive the same product, Certificate of Analysis, and technical support regardless of channel. Contact InVitria or request bulk pricing to place an order.
Storage and Handling
How Should ITSE+A Be Stored?
Store ITSE+A at -20°C (frozen) per the Guidelines for Use and product Certificate of Analysis. Thaw completely before use and handle aseptically when removing aliquots from the primary container. Minimize freeze-thaw cycles to preserve product performance. Contact InVitria technical support for application-specific handling guidance.
Peer-Reviewed Research Citing ITSE+A Animal-Free
ITSE+A Animal-Free has been cited in peer-reviewed publications spanning vaccine development, gene therapy, cell therapy, and clinical diagnostics. Here are the most-cited papers referencing ITSE+A Animal-Free usage, including distribution catalog numbersRelated Resources
| Resource Type | Title | Cell Lines | Relevance |
|---|---|---|---|
| Application Note | Sustained T Cell Proliferation and Early Memory Retention with OptiLeukin™ 2 Recombinant IL-2 | T Lymphocyte, Natural Killer Cells | Demonstrates ITSE+A in defined T-cell expansion media alongside OptiLeukin 2. |
| Application Note | Reducing Serum-Associated Contaminants from Growth Media in Primary Cell Culture | — | Complete ITSE+A serum replacement protocol for primary cell culture. |
| Infographic | Optimizing CAR-T Manufacturing with Defined, AOF Components | T Lymphocyte | Where ITSE+A fits in the CAR-T manufacturing workflow. |