Animal-free recombinant human transferrin for your biomanufacturing and R&D needs
Iron is a requisite component in the biology of life, playing essential roles in cellular metabolism, cell replication, and other, more specialized functions. Free ferrous iron (Fe2+), however, is readily oxidized in solution to its ferric form (Fe3+), which easily catalyzes the formation of toxic reactive oxygen species and free radicals. In the body, this undesirable process is controlled by the iron-binding glycoprotein transferrin, which scavenges extracellular ferric iron in the bloodstream and delivers it back to the cells through endocytosis. Once divested of its payload, iron-free transferrin recirculates to repeat the cycle.
In routine cell culture, transferrin is customarily supplied as a natural component of fetal bovine serum (FBS). The growing use of serum-free, chemically defined media in the culture of cells destined for downstream clinical therapeutic applications, however, necessitates use of a purified, recombinant form of this essential component.
InVitria’s recombinant human transferrin, Optiferrin, is completely free of animal and human components and is produced in a scalable, non-mammalian expression system. It is fully human in primary sequence, is ⍺-glycosylated during expression in the host, and shares secondary and higher-order structural equivalence with naturally occurring human holo-transferrin. Its functional performance in cell culture is equivalent to human holo-transferrin in terms of reversible iron binding, transferrin-receptor binding, and promoting cell growth and productivity.
Optiferrin is naturally free of prion agents and carries a far lower potential risk of contamination with other human or mammalian adventitious agents compared to serum-derived components and recombinant proteins produced in other systems. Its purity makes it ideal for demanding research and commercial applications.
Optiferrin is suitable for a broad range of cell types and applications including:
- Stem, primary, and bioproduction cells
- Robust proliferation of human hematopoietic stem cells (HSC)
- Sp2/0 hybridoma cells
- Any application requiring chemically defined culture media.