Author: Scott Deeter

Process Optimization for Lentivirus Production in the iCELLis® Nano Using a Chemically Defined, Animal Component Free HEK293 Media

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  Abstract Use of FBS in large scale clinical manufacturing applications represents a significant risk due to potential adventitious agent contamination, unreliable supply chain from limited global supply, and considerable variability in vector yield due to lot-to-lot composition variations. To circumvent the many challenges and drawbacks associated with using serum in viral vector production media …

Exbumin – Reconstitution Application Note

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Workflow Summary Weigh Exbumin (lyophilized powder) and determine final total volume of the solution. Add 70% of the final volume of DPBS, PBS, or basal media. Allow the solution to set for 12 hours at 4°C. Adjust the volume to account for volume increase from powder. Sterile filter and store at 2-8°C or freeze at …

OptiVERO – Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media Application Note

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Workflow Summary Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3 to 13 …