Expansion of T Cells without Serum in Chemically Defined Media

{{cta(‘b26cf645-e7f7-4440-b289-6e756fcc2e8d’,’justifyright’)}}Workflow Summary: Combine supplements of OptiPEAK T Lymphocyte media. Isolate human T lymphocytes from whole blood. Wash to remove traces of donor serum. Activate and expand T lymphocytes (7-10 days) in OptiPEAK T Lymphocyte serum free media. Harvest T lymphocytes. Perform post-expansion, flow cytometric analysis of phenotypic surface markers.