Author: jrstaatsiii

Adherent HEK293t Cells Cultured in Pall’s iCELLis® Bioreactor with OptiPEAK HEK293t Blood-Free Chemically Defined Media Exhibit Robust and Rapid Population Doubling Times

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 ICELLIS BIOREACTOR BACKGROUND In the past five years, Pall’s iCELLis bioreactor has emerged as the leading bioreactor technology for clinical and commercial manufacturing of viral vectors and vaccines, with the following data reported in peer- reviewed publications.   Single-use, fixed-bed bioreactor Cell substrate composed of medical grade, uncoated, uncharged polyethylene teraphalate (PET) carriers Closed system …

Expansion of Adherent HEK293T Cells in Flasks in Chemically Defined Cell Culture Media

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Workflow Summary Combine supplements of OptiPEAK HEK293t media. Generate stock solutions and prepare reagents. Adapt HEK293t cells in log phase growth from serum to OptiPEAK HEK293t chemically defined, serum free media. Subculture HEK293t cells every 2-3 days in T-75 flasks, calculating doubling time and population doublings to determine cumulative expansion results. Introduction: For many years, …