Optimizing Antibody Production in Animal-Free Media with the Supplementation of Recombinant Human Albumin (Cellastim™)

Published on 9 September 2020

Abstract

Due to the increased risk of transmitting infectious agents, antibody production is moving away from the use of animal-derived components in bioproduction. The switch to animal-free cell culture media often results in less performance which is evident in longer doubling time, lower peak cell density and reduced antibody titer. We evaluated recombinant human albumin (rAlbumin, Cellastim), produced in a non-animal plant host, for its effect in hybridoma cell culture. Cellastim outperformed plasma-derived HSA (pHSA) in promoting cell growth and antibody titer. The use of Cellastim resulted in an average 140% increase in cell growth and 92% increase in volumetric antibody productivity across several media formulations. Cellastim improved the growth of cells in long term culture. In addition, Cellastim improved the effect of serum withdrawal and eased the transition to chemically defined media.

 

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