Reducing Serum-Associated Contaminants from Growth Media in Primary Cell Culture
Workflow Summary Supplement basal media with ITSE + A to a final 1x-2x concentration. Reduce typical percentage of added FBS by 50-90%. Expand cells as normal in ITSE + A supplemented media for desired expansion time. Perform post-expansion cell count and doubling-time calculation. Introduction Fetal bovine serum contains an abundance of critical proteins and components …