Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media

{{cta(‘1784143b-e10e-4e9c-a96b-547fe26397a1′,’justifyright’)}}Workflow Summary: Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus …