Abstract Due to the increased risk of transmitting infectious agents, antibody production is moving away from the use of animal-derived components in bioproduction. The switch to animal-free cell culture media often results in less performance which is evident in longer doubling time, lower peak cell density and reduced antibody titer. We evaluated recombinant human albumin …
Introduction ITSE Animal-Free is a recombinant, animal-free media supplement used to improve cell performance. ITSE Animal-Free provides nutrients that are required for cell growth, and replaces other ITS or ITSE supplements that have components derived from animals. ITSE Animal-Free is an IgG-free sterile liquid, making it simple to use and allows easier purification of protein …
Due to the multifaceted biological role of human serum albumin, inclusion in cell culture media has been shown to be extremely beneficial for the propagation of many cell types ex vivo. However, proteins isolated from human serum can have variable performance and potential adventitious agent contamination risks. Thus, the investigation into the inclusion of a recombinant …
Abstract The measurement of the electrophysiology of human pluripotent stem cell-derived cardiomyocytes is critical for their biomedical applications, from disease modeling to drug screening. Yet, a method that enables the high-throughput intracellular electrophysiology measurement of single cardiomyocytes in adherent culture is not available. To address this area, we have fabricated vertical nanopillar electrodes that can …
