Worldwide, researchers and industry leaders are improving health outcomes for patients by utilizing the multi-functional efficacy of regulatory t cells to develop novel therapies and disease treatments. Regulatory T cells, commonly referred to as ‘T-regs’, play a critical role in maintaining order in the human immune system by regulating and suppressing excessive inflammation. Given the therapeutic …
The number and breadth of applications for induced human embryonic and pluripotent stem cells (hi-ESCs and hi-PSCs) in both research and clinical settings continue to co-evolve with our understanding of how to harness them as biological tools. In the field of cardiology, for example, it has been suggested that the ability to derive functional human …
The American Society of Gene & Cell Therapy (ASGCT) held their 22nd annual meeting in Washington D.C. last week from April 29th through May 2nd bringing together a record number of over 3,400 attendees to share and discuss the remarkable work going on to advance this field. With 23 Scientific Symposia and 1000 abstracts presented …
Enhanced liquid-phase stability of live-attenuated Flavivirus vaccines using recombinant albumin The single-stranded RNA viruses belonging to the genus Flavivirus (family, Flaviviridae) are significant to global public health due to the high rates of morbidity and mortality they cause.1 For example, Yellow Fever sickens approximately 200,000 people and kills 30,000 annually.2 Other viruses belonging to this …
Workflow Summary Combine supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add …
Workflow Summary Supplement basal media with ITSE + A to a final 1x-2x concentration. Reduce typical percentage of added FBS by 50-90%. Expand cells as normal in ITSE + A-supplemented media for desired expansion time. Perform post-expansion cell count and doubling-time calculation. Introduction Fetal bovine serum contains an abundance of critical proteins and components that …
Combine supplements of OptiVERO Serum Free Media (SFM). Prepare complementary reagents. Wash cells to remove traces of donor serum. Remove cells from growth surface. Wash and collect cells. Centrifuge. Resuspend. Count. Seed in OptiVERO SFM. Introduction Vero cells derived from the African green monkey are one of the most common immortalized mammalian cells lines used …
On Tuesday, the vaccine company FluGen announced promising preliminary results from a placebo-controlled, Phase 2 clinical trial of their investigational intranasal influenza vaccine, M2SR, in 99 healthy adults challenged with the A/Belgium/4217/2015, H3N2 strain.
In January, there’s no better place to be than Miami, FL. While we were excited to escape the cold winter, it was all business for InVitria during Phacilitate 2019 and the Regenerative Medicine Foundation’s World Stem Cell Summit. This year, there was an excitement in the air due to the significant increase in the number of cell therapies in the clinical pipeline. With some …
