Author: Patrick Morgan
Virus Yield Improvement for Downstream Processing: Exbumin®, Excipient Recombinant Albumin
Introduction Biological manufacturers face many challenges in downstream processing unit operations of viral based therapeutics that impact the integrity, stability, and potency of their final drug product. Human Serum Albumin (HSA) has been shown to improve yield during common downstream purification unit operations such as tangential flow filtration, depth filtration, chromatography, sterile filtration, and final …
Transient Transfection Protocol – Enhancement of Transfection Efficiency Using Recombinant Transferrin with Serum-Free HEK293 Media
Enhancement of Transient Transfection with Serum-Free and Blood-Free Transferrin Abstract Methods to enhance transient transfection of adherent cells have identified many different ligands that improve plasmid uptake into cells. Of these ligands, serum-derived transferrin is well-known to improve transfection efficiency of adherent cells. However, to date, a serum-free and blood-free transferrin has not been …