ITS AF^™ – Blood-free cell culture media supplement

Product Description

ITS Animal-Free™ (ITS AF) is a 100x recombinant insulin and transferrin cell culture supplement, optimized for serum-free and reduced-serum media applications. Designed to enhance cell growth, metabolic activity, and viability, ITS AF provides a chemically defined, blood-free alternative to traditional insulin-transferrin-selenium (ITS) supplements.

Unlike ITSE, ITS AF contains three essential components required for optimal cell growth in serum-free and reduced-serum culture media, but lacks ethanolamine, making it ideal for applications requiring a simpler, serum-free formulation.

Manufactured in an ISO 9001:2015-certified, cGMP-compliant facility in the United States, ITS AF ensures batch-to-batch consistency, pathogen safety, and scalability for biopharmaceutical, cell therapy, and regenerative medicine applications.

Advantages

• Optimized for Serum-Free, Animal-Origin-Free Cell Culture
  • Fully recombinant insulin and transferrin, providing a stable and defined supplement for cell culture.
  • Enhances cell proliferation, metabolic activity, and viability across multiple cell types.
  • Optimized for chemically defined media, supporting serum-free and reduced-serum applications.
• Animal-Origin-Free Assurance
  • 100% chemically defined and animal-origin-free (AOF) – eliminating batch variability and regulatory risks associated with serum-derived supplements.
  • Manufactured using a non-animal, non-human host system, ensuring purity and reproducibility.
  • Raw materials, manufacturing equipment, and packaging are fully animal-free, aligning with regulatory and ethical standards.
• Manufactured in the United States
  • Produced in a tertiary-level, ISO 9001:2015-certified facility in the USA.
  • Complete supply and manufacturing chain is owned and operated by InVitria, ensuring unmatched traceability and quality control.
  • Batch-to-batch consistency for reliable integration into cGMP cell culture workflows.
• Ready-to-Use 100x Sterile Liquid Format
  • Supplied as a 100x sterile liquid, allowing for precise supplementation in cell culture media.
  • Blood-free components improve performance consistency, ensuring reproducibility across experiments.
  • Cost-effective formulation, reducing dependence on costly serum supplementation.

Applications

• Serum-Free & Reduced-Serum Cell Culture
  • Supports CHO, HEK, VERO, and stem cell culture in chemically defined media.
  • Enhances cell viability and proliferation without the need for fetal bovine serum (FBS).
• Cell Therapy & Regenerative Medicine
  • Provides a chemically defined alternative to serum-derived insulin and transferrin.
  • Ensures greater control over cell culture conditions for stem cell and immune cell expansion.
• Biopharmaceutical & Vaccine Production
  • Improves cell productivity in protein and viral vector production workflows.
  • Enhances growth and metabolic stability in CHO and HEK-based biomanufacturing.

Technical Specifications

• Formulation: 100x sterile liquid cell culture supplement
• Manufacturing: Produced in a state-of-the-art, ISO 9001:2015-certified facility in the USA
• Quality Assurance:
  • Fully recombinant and animal-origin-free production system
  • Stringent quality control processes ensure high batch-to-batch consistency
• Regulatory Compliance & Safety:
  • cGMP-compliant manufacturing
  • Plasma-free, pathogen-free, and chemically defined
  • Aligned with regulatory expectations for biopharmaceutical, regenerative medicine, and vaccine production applications

Why Choose ITS Animal-Free?

ITS Animal-Free is a cost-effective, chemically defined alternative to traditional ITS supplements, offering unparalleled purity, stability, and regulatory compliance. Whether used for serum-free biomanufacturing, cell therapy, or regenerative medicine, ITS AF provides a safe, scalable, and animal-free solution for industrial and research applications.

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