ITSE Animal-Free^® – Blood-free cell culture media supplement

Product Description

ITSE Animal-Free® (ITSE AF) is a 100x recombinant insulin, transferrin, selenium, and ethanolamine cell culture supplement, optimized for serum-free and reduced-serum media applications. This fully recombinant, chemically defined formulation provides essential components to support cell growth, metabolic function, and oxidative stress protection, making it ideal for CHO, HEK, VERO, and stem cell culture.

Designed to replace undefined serum-derived supplements, ITSE AF improves cell performance, consistency, and viability across a range of biopharmaceutical, cell therapy, and regenerative medicine applications. Manufactured in an ISO 9001:2015-certified, cGMP-compliant facility in the United States, ITSE AF ensures batch-to-batch consistency, regulatory compliance, and pathogen-free safety.

Advantages

• Optimized for Serum-Free, Animal-Origin-Free Cell Culture
  • Fully recombinant, blood-free supplement, eliminating batch variability associated with serum-derived ITS supplements.
  • Enhances cell proliferation, metabolic activity, and viability in chemically defined media.
  • Supports growth in a wide range of cell types, including CHO, HEK, and primary cells.

• Essential Components for Cell Growth & Performance
  • Insulin – Promotes cell signaling, glucose uptake, and amino acid transport for enhanced metabolic activity.
  • Transferrin – Provides a non-toxic iron carrier, preventing oxidative stress and free-radical formation.
  • Selenium – Required for the activity of antioxidant enzymes, protecting cells from oxidative damage.
  • Ethanolamine – A phospholipid precursor, essential for membrane integrity and the growth of select cell types.
• Animal-Origin-Free Assurance
  • 100% chemically defined and animal-origin-free (AOF) – eliminating risks associated with serum-derived components.
  • Manufactured using a non-animal, non-human host system, ensuring purity and reproducibility.
  • Raw materials, manufacturing equipment, and packaging are fully animal-free, aligning with regulatory and ethical standards.
• Manufactured in the United States
  • Produced in a tertiary-level, ISO 9001:2015-certified facility in the USA.
  • Complete supply and manufacturing chain is owned and operated by InVitria, ensuring unmatched traceability and quality control.
  • Batch-to-batch consistency for reliable integration into cGMP cell culture workflows.
• Ready-to-Use 100x Sterile Liquid Format
  • Supplied as a 100x sterile liquid, allowing for precise supplementation in cell culture media.
  • Blood-free components improve performance consistency, ensuring reproducibility across experiments.
  • Cost-effective formulation, reducing dependence on costly serum supplementation.

Applications

• Serum-Free & Reduced-Serum Cell Culture
  • Supports CHO, HEK, VERO, and stem cell culture in chemically defined media.
  • Enhances cell viability and proliferation without the need for fetal bovine serum (FBS).
• Cell Therapy & Regenerative Medicine
  • Provides a chemically defined alternative to serum-derived insulin and transferrin.
  • Ensures greater control over cell culture conditions for stem cell and immune cell expansion.
• Biopharmaceutical & Vaccine Production
  • Improves cell productivity in protein and viral vector production workflows.
  • Enhances growth and metabolic stability in CHO and HEK-based biomanufacturing.

Technical Specifications

• Formulation: 100x sterile liquid cell culture supplement
• Manufacturing: Produced in a state-of-the-art, ISO 9001:2015-certified facility in the USA
• Quality Assurance:
  • Fully recombinant and animal-origin-free production system
  • Stringent quality control processes ensure high batch-to-batch consistency
• Regulatory Compliance & Safety:
  • cGMP-compliant manufacturing
  • Plasma-free, pathogen-free, and chemically defined
  • Aligned with regulatory expectations for biopharmaceutical, regenerative medicine, and vaccine production applications

Why Choose ITSE Animal-Free?

ITSE Animal-Free is a cost-effective, chemically defined alternative to traditional ITS supplements, offering unparalleled purity, stability, and regulatory compliance. Whether used for serum-free biomanufacturing, cell therapy, or regenerative medicine, ITSE AF provides a safe, scalable, and animal-free solution for industrial and research applications.

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