Author: Alexia Armstrong

Improved iPSC Cryopreservation with Optibumin 25: Enhanced Viability, Expansion, and Pluripotency

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Overview This application note evaluates the performance of Optibumin® 25, a recombinant, animal-origin-free human serum albumin (rHSA), in the cryopreservation of human induced pluripotent stem cells (iPSCs). The study demonstrates that Optibumin 25 enables a significant reduction in DMSO concentration while improving post-thaw viability, expansion, and pluripotency. Compared to blood-derived HSA, Optibumin 25 delivers superior …

Superior Cryopreservation of T Cells in Low-DMSO, Animal-Origin-Free Formulation Using Optibumin 25 and PentaHibe Base

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Overview This application note presents a defined, animal-origin-free (AOF) cryopreservation strategy addressing the limitations of high-DMSO and serum-derived components. The formulation—10% PentaHibe Base, 2% DMSO, and 4% Optibumin 25—was tested in CAR-T–mimicking workflows using healthy donor T cells. PentaHibe Base is a non-penetrating, carbohydrate-based cryoprotectant derived from pentaisomaltose, a patented subfraction of Dextran 1 with …

Optibumin 20 – Guidelines for Use

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Download the Optibumin 20 Guidelines for Use to learn preparation, concentrations, storage, and applications of recombinant HSA. High free‑thiol Cys‑34 content and 99% fatty‑acid‑free profile support small‑molecule solubilization, bioconjugation, and sensitive applications including iPSC, hESC, and IVF.

Lacromin – Guidelines for Use

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Download the Lacromin® Guidelines for Use to learn preparation, concentrations, storage, and applications of recombinant human lactoferrin. This animal-origin-free supplement supports CHO, HEK, pluripotent, and select primary cell cultures.

LIF – Guidelines for Use

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Download the LIF Guidelines for Use to learn preparation, dosing, storage, and applications of recombinant human LIF. Animal-origin-free and chemically defined, it supports neural stem cell expansion and select pluripotent stem cell workflows.

OptiLeukin 2 – Guidelines for Use

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Download the OptiLeukin 2 Guidelines for Use to learn preparation, dosing, storage, and applications of animal‑ and bacteria‑origin‑free recombinant IL‑2. Designed for GMP‑aligned workflows in T cell manufacturing and research.

Optibumin 25 – Guidelines for Use

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Download the Optibumin® 25 Guidelines for Use to learn preparation, concentrations, storage, and applications of recombinant human serum albumin. Manufactured animal-origin-free, it supports safer, scalable workflows in cell therapy, vaccine, and biologics manufacturing.

In Memory of Dr. William J. Rutter (1927–2025)

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We are deeply saddened by the passing of Dr. William J. Rutter—a visionary scientist, mentor, entrepreneur, and close friend to many at InVitria. He passed away on July 11, 2025, at the age of 97. Few scientists have shaped modern biotechnology as deeply as Dr. Rutter. His research laid the groundwork for recombinant insulin, genetically …

ITS-Animal-Free™ Supplementation for iPSC Expansion and Pluripotency: A Platform Approach for Serum-Free Cell Expansion

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Overview ITS Animal-Free™ ITS Animal-Free (ITS AF), InVitria’s 100x cocktail of Insulin, Transferrin, and Selenium that is completely animal-origin-free, is essential for culture of all mammalian cell types including iPSCs. ITS AF can be conveniently supplemented into custom media formulations to replace the core components of serum. This reduces variability, eliminates safety and regulatory hurdles, …

Safe, Consistent Iron Delivery in Serum-Free Systems with Optiferrin® Recombinant Transferrin

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Overview Efficient iron delivery plays a critical role in maintaining cell health, supporting proliferation, and maximizing therapeutic productivity. However, serum-derived transferrin introduces variability, regulatory risk, and the potential for contamination. This application note demonstrates how Optiferrin, a recombinant transferrin for iron delivery in serum-free media, provides a consistent and animal-origin-free alternative. It mirrors native transferrin …